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Brand Name : REAGEN
Model Number : RNA97008
Place of Origin : USA
MOQ : 5 kits
Price : Negotiable
Payment Terms : T/T
Supply Ability : 100 kits per month
Delivery Time : 5-7 days
Packaging Details : color packing
Type : reagent
Size : 96 test
Package : color packing
Shelf life : one year
Sensitivity : 0.05ppm
stored : 2-8 degrees
Okadaic Acid(DSP)ELISA Test Kit
REAGEN™Okadaic Acid (DSP) ELISA Test Kit is a competitive enzyme immunoassay for the quantitative analysis of Okadaic Acid (DSP) in mussels and water samples.
1.High recovery (80-105%), rapid (10-30 minutes), and cost-effective extraction methods
2.High sensitivity (0.3 ng/g or ppb) and low detection limit (30 ppb for mussel samples and 6 ppb for water samples).
3.High reproducibility.
4.A quick ELISA assay(less than 1.5 hours regardless of number of samples).
The method is based on a competitive colorimetric ELISA assay. The second antibody against Anti-Okadaic Acid Antibody was coated in the plate wells. During the analysis, sample is added along with the okadaic acid-horseradish peroxidase conjugate (Okadaic Acid-HRP Conjugate) and Anti- Okadaic Acid Antibody. If the okadaic acid residue is present in the sample, it will compete with Okadaic Acid-HRP Conjugate for the Anti-Okadaic Acid Antibody, thereby preventing the Okadaic Acid-HRP Conjugate from binding to the Anti-Okadaic Acid Antibody. The Anti-Okadaic Acid Antibody is then bound by the second antibody immobilized on the plate. After a washing step and addition of the TMB substrate solution, a color signal is produced. The color reaction is stopped after a specified time and the color is evaluated using an ELISA reader. The resulting color intensity has an inverse relationship with the okadaic acid residue concentration in the sample.
Sample Type | Detection Limit (ng/g or ppb) |
Mussels | 30 |
Water | 6 |
Analytes | Cross-Reactivity (%) |
Okadaic Acid | 100.0 |
DTX1 | 40 |
DTX2 | 46 |
Neosaxitoxin | < 0.01 |
Saxitoxin | < 0.01 |
YTX | < 0.01 |
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